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An experiment was conducted to determine the effects of nano-copper at different levels (0, 14, 16, and 18 ppm respectively) as antibacterial against E.coli bacteria. Each group of 100 eggs was injected with E. coli bacteria (1 m × 10-7) and was exposed to 38°C in the hatcher. Significant increase was observed in G1 for the E. coli and total count bacteria. There was significant improvement for G5 in abnormal chicks with significant improvement for G2, G3, and G4 in addled eggs, live pipped chicks and dead pipped chicks. Significant improvement was also observed for nano copper groups in activity, general situation, appearance and feather condition, case of yolk retracted inside the abdomen, navel case and residual yolk membrane. There was a significant decrease in cholesterol, glucose, and triglyceride for the G2, G3, and G4 groups and a significant increase for the G2 group in GSH. At same time, a significant decrease in MDA in the G2 and G3 groups and a significant increase in AST enzyme for the G1 group.

Keywords: Antibacterial, nanotechnology, copper, hatching, Japanese quail