open access


This study investigated the effect of injecting hatching eggs with different anthocyanin concentrations on the morphological and physiological characteristics of one-day hatching chicks at two different times (days 12 and 18) of the incubation period. This study was conducted at Al-Anwar hatchery facility from 11-11-2023 to 2-12-2023. A total of 900 fertilized eggs were used for this study, divided into two groups (450 eggs each), and each group had six treatments with three replicates and 25 eggs for each replicate. The treatments were (NC) negative control, (PC) positive control (NaCl solution), and 50, 60, 70, and 80 ppm of Anthocyanin for T3, T4, T5, and T6, respectively. Results showed that hatching and embryonic mortality percentages were significantly affected in both injected dates in addition to the hatched chick weight in the second period. The percentage of dead piped chicks was significantly low on the 50-ppm anthocyanin level compared to other treatments on day 18 while no significant effect was found on day 12 of the incubation period. The highest recorded chick length was at the level of 50 ppm anthocyanin compared to the control on day 18, while on day 12, the 60 ppm anthocyanin level significantly showed the highest wing length value compared to the control. Both leg length and tonic immobility were significantly affected in both periods, especially when the anthocyanin treatments significantly increased leg length and decreased tonic immobility compared to the control treatments of the second incubation period. Significant differences were obtained between treatments on the appearance and feather condition, leg, and navel status at day 12, while at day 18, only chick activity and appearance and feather condition significantly appeared different effects between treatments. 

Keywords: Anthocyanin, hatching chicks, morphological traits, in-ovo feeding